[HTML][HTML] Calcium-induced transformation of cardiolipin nanodisks

CA Fox, P Ellison, N Ikon, RO Ryan - Biochimica et Biophysica Acta (BBA) …, 2019 - Elsevier
… CL ND were analyzed by gel filtration chromatography as described above before and after
… The plate was incubated for 1 h at 22 C followed by sample absorbance determination at …

Effects of phosphorylation on Drp1 activation by its receptors, actin, and cardiolipin

A Liu, AL Hatch, HN Higgs - Molecular Biology of the Cell, 2024 - Am Soc Cell Biol
… We use a concentration of Mff (50 μM) that causes a … (C) Superose 6 gel filtration profiles of
WT and P-S579-Drp1. At … in a manner that allows determination of interaction affinity. Finally, …

Studies of the cardiolipin interactome

CA Fox, RO Ryan - Progress in Lipid Research, 2022 - Elsevier
Gel filtration chromatography of the clarified sample revealed … and underwent a symptom
assessment evaluation. While … were observed in both symptom assessment scores and a 6-min …

[PDF][PDF] Cryo-EM grid optimization for membrane proteins

D Kampjut, J Steiner, LA Sazanov - Iscience, 2021 - cell.com
… bottleneck in protein structure determination, especially for … by cation exchange
chromatography and gel filtration, while … proteins and 1:4 cardiolipin:dioleoyl phosphatidylcholine …

Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer

A Panda, F Giska, AL Duncan, AJ Welch, C Brown… - Nature …, 2023 - nature.com
… To obtain the best experience, we recommend you use a more up to date browser (or turn …
This sample was placed on top of the prepared column and separated through gel filtration to …

Structural insights into cardiolipin replacement by phosphatidylglycerol in a cardiolipin-lacking yeast respiratory supercomplex

CF Hryc, VKPS Mallampalli, EI Bovshik… - Nature …, 2023 - nature.com
… To obtain the best experience, we recommend you use a more up to date browser (or turn off
… Our structural determination of the trimeric SC from CRD1∆ cells shows the presence of the …

PB1F2 from influenza a virus regulates the interaction between cytochrome C and cardiolipin

Y Wang, J Wang - Membranes, 2022 - mdpi.com
… Based on this, we use two kinds of membrane mimic system … Finally, a Superdex 200 column
with gel filtration was used to … The determination of the MIP can provide information on the …

Cardiolipin promotes pore-forming activity of alpha-synuclein oligomers in mitochondrial membranes

S Ghio, A Camilleri, M Caruana, VC Ruf… - ACS chemical …, 2019 - ACS Publications
estimates of 0.57 nm (Open-1, 100 pS), 0.82 nm (Open-2, 200 pS), and 1.18 nm (Open-3,
400 pS). … In summary, we demonstrated that use of antiaggregator molecules could effectively …

Formulation and Characterization of Bioactive Agent Containing Nanodisks

K Lethcoe, CA Fox, I Moh, M Swackhamer… - JoVE (Journal of …, 2023 - jove.com
… Characterization studies involve the determination of … liquid chromatography (FPLC) gel
filtration chromatography is … of an accessible cardiolipin bilayer 8 . Based on these, and other …

Optimizing purification of the peripheral membrane protein FAM92A1 fused to a modified spidroin tag

Z Wang, C Mim - Protein Expression and Purification, 2022 - Elsevier
… for structure determination. Here, we present an optimized … We used analytical gel filtration
to further characterize and … We added each protein to liposomes containing cardiolipin. We …